Center of Excellence on Environmental Health and Toxicology (EHT)
| Bisphenol A (BPA) is an endocrine disrupting chemical (EDCs) that widely distribute in aquatic environment and has a great impact on aquatic wildlife. In this study, 3-month-old clown anemonefish (Amphiprion ocellaris) were studied for a biological indicators of BPA contamination in the coastal area of Map Ta Phut Industral Estrate zone, Rayong Province. The fish had been exposed to BPA at 100 ng/L for 6 hours and measured BPA accumulation in the liver and muscle by high-performance liquid chromatography (HPLC). The results showed that BPA accumulation in the liver (116.52 ± 3.15 ng/g) was significantly higher than the muscle (54.49 ± 1.98 ng/g). To determine the level of cytochrome P450 1A (cyp1a) gene expression by SYBR Green real-time PCR, the anemonefish were exposed to 50 and 100 ng/L of BPA for 0, 6, 12, 24, and 48 hours, in comparison to the control group (no BPA). The results showed that the level of cyp1a expression was highest (3.44-fold) when the fish exposed to 100 ng/L for 12 hours and 50 ng/L for 24 hours (2.84-fold). However, when the 6-month-old fish exposed to BPA contaminated seawater (246 ± 18 ng/L) collected from Ao Pradu at Map Ta Phut Industrial Estate area for 3 hours, the level of cyp1a expression of the fish was slightly higher (1.41 ± 0.22-fold) but not significantly different from the control group. Therefore, the cyp1a gene and anemonefish can be used as a biomarker of BPA exposure and a studying model species of the BPA effects on wildlife.
แหล่งข้อมูล:
|
